Title:The Tully-Fisher relation as a tool to investigate the internal structure of galaxies Abstract: To investigate the internal structure of galaxies we consider in detail the 3.6 μm Tully-Fisher relation taking advantage of spatially resolved HI velocity fields. The main idea is to abandon the classical concept of the Tully-Fisher relation as the correlation using the width of global HI profile, and consider instead the detailed internal kinematics of gas in galaxies. An improved kinematic measure is implemented by deriving hidh-quality rotation curves from the velocity fields, taking into account warps and streaming motions in the disk due to spiral arms or a bar. The decomposition of HI rotation curves into components will provide the mass distribution within the galaxies including baryonic and dark matter.