Oral title: CO depletion on rotational line profiles from various CO isotopologues Oral abstract: Our chemical evolution models indicate that the CO abundance is a strong function of time. The abundance of CO decreases because of the formation of complex organic molecules, causing a mass-age degeneracy, as well as producing an effect that can be misinterpreted as the “snow line”. In this talk, I will discuss the impact of CO depletion on rotational line profiles from various CO isotopologues.